RESUMO
Our study was designed to test the hypothesis that an early morning sputum may be sufficient for calculation of early bactericidal activity (EBA). Patients underwent sputum collection randomly (spot, 5â¯h and 12â¯h) in consecutive days. The median CFU count in the spot samples group was 5.67 log10 CFU/mL compared to 6.17 log10 CFU/mL in 5â¯h and 6.23 log10 CFU/mL in 12â¯h samples. Inter-patient comparison showed low coefficient of variation for both 12â¯h (11%) and 5â¯h samples (10%). Intrapatient samples analysis demonstrated that the median bacillary load variation (0.037 log10 CFU/mL and 0.022 log10 CFU/mL for 5 and 12â¯h samples respectively) was comparable to the other EBA studies and did not vary significantly from one day of collection to another. We concluded that 5â¯h pooled sputum when collected appropriately in the morning can be sufficient for calculation of EBA.
Assuntos
Bactérias/isolamento & purificação , Variação Biológica da População/fisiologia , Manejo de Espécimes/métodos , Escarro/microbiologia , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto JovemRESUMO
ABSTRACT Tuberculosis is an infectious disease of global importance with major economic and social burden accounting for 25% of all avoidable deaths in developing countries. Extrapulmonary involvement may occur either in association with clinically apparent pulmonary tuberculosis or in isolation. This cross-sectional descriptive study aimed to evaluate the impact of ocular tuberculosis in visual acuity at baseline and after two months of intensive anti-tuberculous therapy. A sample of 133 pulmonary tuberculosis patients, seven disseminated tuberculosis, and three pleural tuberculosis patients was evaluated. All patients underwent routine ophthalmic evaluation, including assessment of visual acuity, biomicroscopy, applanation tonometry, indirect ophthalmoscopy, and fluorescent angiography as appropriate. None of the patients had impaired visual acuity due to tuberculosis. A rate of 4.2% (6/143) of ocular involvement was found. None of the patients with ocular involvement were HIV-infected. Of the six patients with ocular involvement, five met the diagnostic criteria for probable and one for possible ocular lesions. As for the type of ocular lesions, two patients had bilateral findings: one had sclerouveitis and the second had choroidal nodules. The other four patients presented with unilateral lesions: peripheral retinal artery occlusion in the right eye (one case), choroidal nodules in the left eye (one case), and choroidal nodules in the right eye (two cases). Patients progressed favorably after two month of intensive therapy, with no significant reduction in vision.
Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , Adulto Jovem , Visão Ocular/fisiologia , Acuidade Visual/fisiologia , Tuberculose Ocular/fisiopatologia , Tuberculose Ocular/tratamento farmacológico , Antituberculosos/uso terapêutico , Pirazinamida/uso terapêutico , Rifampina/uso terapêutico , Transtornos da Visão/fisiopatologia , Transtornos da Visão/microbiologia , Tuberculose Ocular/complicações , Estudos Transversais , Resultado do Tratamento , Estatísticas não Paramétricas , Etambutol/uso terapêutico , Isoniazida/uso terapêuticoRESUMO
Tuberculosis is an infectious disease of global importance with major economic and social burden accounting for 25% of all avoidable deaths in developing countries. Extrapulmonary involvement may occur either in association with clinically apparent pulmonary tuberculosis or in isolation. This cross-sectional descriptive study aimed to evaluate the impact of ocular tuberculosis in visual acuity at baseline and after two months of intensive anti-tuberculous therapy. A sample of 133 pulmonary tuberculosis patients, seven disseminated tuberculosis, and three pleural tuberculosis patients was evaluated. All patients underwent routine ophthalmic evaluation, including assessment of visual acuity, biomicroscopy, applanation tonometry, indirect ophthalmoscopy, and fluorescent angiography as appropriate. None of the patients had impaired visual acuity due to tuberculosis. A rate of 4.2% (6/143) of ocular involvement was found. None of the patients with ocular involvement were HIV-infected. Of the six patients with ocular involvement, five met the diagnostic criteria for probable and one for possible ocular lesions. As for the type of ocular lesions, two patients had bilateral findings: one had sclerouveitis and the second had choroidal nodules. The other four patients presented with unilateral lesions: peripheral retinal artery occlusion in the right eye (one case), choroidal nodules in the left eye (one case), and choroidal nodules in the right eye (two cases). Patients progressed favorably after two month of intensive therapy, with no significant reduction in vision.